Quarantine - How To
“...quarantine remains the responsibility of the supply chain.”
How to Quarantine Effectively
While it is the responsibility of the industry to quarantine, it is every keeper's best practice procedure too . You can always blame someone, but you can never get your fish back to life.
Please also note that KHV is now termed a "re-portable disease" and you are obligated by law to inform your local health authorities who will ensure that the correct procedure is followed. PLEASE NOTE.
The following procedure is what we regards as MILD and absolutely MINIMUM for efficient quarantining against KHV. For other viral diseases different procedure may be required.
Week 1 and 2 with 0.3% salt always in Q-tank
Day 1 – Inspect the fish for obvious transport damage.
Day 2 – Observe, “scrape” and check microscopically, Treat immediately if required correctly
Raise water temperature to 23oC ± 3o . It is possible to combine day 1 & 2 into one.
Day 3 – Subject all the fish to Standard Physiological Stress
Day 4-10 Wait, observe fish for 7 days
Day 10 – Sample for KHV at 95% confidence . Pool samples from the same farm.
Day 11-14 - Wait for lab results
Day 14 – Introduce if no illness observed or tested (can give potassium permanganate dip)
If your fish traveled badly make sure gill damage is not observed due to lack of oxygen. Wait till the fish are healthy before the KHV testing cycle.
Let me just tell you. If you find the fish showing signs of illness by Day 5-10 after the salt dip, you have a high probability of KHV. Then one sample per batch is sufficient to confirm.
Following is what we term a Standard Physiological Stress Test. If the ambient temperature and incubation period are observed, this will almost always bring on a latent disease condition that can be tested. Test for SVC at 17oC and KHV at 23oC
Standard Physiological Stress:
Dip the fish for 2 minutes in 30 g per liter salt solution (NaCl) - Remove fish if it flips over or cannot cope with the treatment!
Alternatively: You can also do a 7 minutes dip in 1 g KMnO4 per 10 liter. It was found to have the same effect and/can be used if treatment of certain parasites is also required.
It is no use to pay for testing at a lab if you take a POOR SAMPLE under the wrong conditions. Fish are not on medical aid to absorb the costs of re-testing and soothing you conditions is false security.
It is absolutely critical that all fish are quarantined and samples tested before integrating with your collection. Keep fish from different farms or locations separately until AFTER quarantine.
Work biosecure and diligently keep record of the progress.
Do not mix the water from the different groups under test
Servaas de Kock